Disruption of the Membrane Nuclease Gene (MBOVPG45_0215) of Mycoplasma bovis Greatly Reduces Cellular Nuclease Activity
Shukriti Sharma, Kelly A Tivendale, Philip F Markham, Glenn F Browning
Journal of Bacteriology | AMER SOC MICROBIOLOGY | Published : 2015
UNLABELLED: Although the complete genome sequences of three strains of Mycoplasma bovis are available, few studies have examined gene function in this important pathogen. Mycoplasmas lack the biosynthetic machinery for the de novo synthesis of nucleic acid precursors, so nucleases are likely to be essential for them to acquire nucleotide precursors. Three putative membrane nucleases have been annotated in the genome of M. bovis strain PG45, MBOVPG45_0089 and MBOVPG45_0310, both of which have the thermonuclease (TNASE_3) functional domain, and MBOVPG45_0215 (mnuA), which has an exonuclease/endonuclease/phosphatase domain. While previous studies have demonstrated the function of TNASE_3 domain..View full abstract
S.S. was supported by an AusAid scholarship (now Australia Award) provided by the Australian Department of Foreign Affairs and Trade.