Journal article
Analysis of the Genome and Transcriptome of Cryptococcus neoformans var. grubii Reveals Complex RNA Expression and Microevolution Leading to Virulence Attenuation
G Janbon, KL Ormerod, D Paulet, EJ Byrnes, V Yadav, G Chatterjee, N Mullapudi, CC Hon, RB Billmyre, F Brunel, YS Bahn, W Chen, Y Chen, EWL Chow, JY Coppée, A Floyd-Averette, C Gaillardin, KJ Gerik, J Goldberg, S Gonzalez-Hilarion Show all
Plos Genetics | PUBLIC LIBRARY SCIENCE | Published : 2014
Open access
Abstract
Cryptococcus neoformans is a pathogenic basidiomycetous yeast responsible for more than 600,000 deaths each year. It occurs as two serotypes (A and D) representing two varieties (i.e. grubii and neoformans, respectively). Here, we sequenced the genome and performed an RNA-Seq-based analysis of the C. neoformans var. grubii transcriptome structure. We determined the chromosomal locations, analyzed the sequence/structural features of the centromeres, and identified origins of replication. The genome was annotated based on automated and manual curation. More than 40,000 introns populating more than 99% of the expressed genes were identified. Although most of these introns are located in the cod..
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Awarded by National Human Genome Research Institute
Funding Acknowledgements
This work was supported by the National Human Genome Research Institute, grant number U54HG003067 to the Broad Institute. This project has been funded in whole or in part with Federal funds from the National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services, under Contract No.: HHSN272200900018C. This work was supported by a grant from ANR (2010-BLAN-1620-01 program YeastIntrons) to GJ. This work was also supported by NIH/NIAID R37 award AI39115-16 and R01 award AI50113-10 (JH), NIH/NIAID R21 award AI094364 (AI), and by NIH/NINDS R01 grant NS042263 (FSD). The intramural support of JNCASR to KS for the centromere work is highly acknowledged. The work on replication origins was funded by a Burroughs Wellcome Scholar Award in Molecular Pathogenic Mycology awarded to CSN. The work on microevolution was funded by the NHMRC Project Grant 455980 (JAF). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.