Journal article

A gene for carotene cleavage required for pheromone biosynthesis and carotene regulation in the fungus Phycomyces blakesleeanus

Victor G Tagua, Humberto R Medina, Raul Martin-Dominguez, Arturo P Eslava, Luis M Corrochano, Enrique Cerda-Olmedo, Alexander Idnurm

FUNGAL GENETICS AND BIOLOGY | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2012

Abstract

Mating and sexual development in fungi are controlled by molecular mechanisms that are specific for each fungal group. Mating in Phycomyces blakesleeanus and other Mucorales requires pheromones derived from β-carotene. Phycomyces mutants in gene carS accumulate large amounts of β-carotene but do not enter the sexual process. We show that carS encodes a β-carotene-cleaving oxygenase that catalyzes the first step in the biosynthesis of a variety of apocarotenoids, including those that act as pheromones. Therefore carS mutants cannot stimulate their sexual partners, although they respond to them. CarS catalyzes the biosynthesis of a β-ring-containing apocarotenoid that inhibits the activity of ..

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University of Melbourne Researchers

Grants

Awarded by US National Science Foundation


Awarded by Spanish grants


Funding Acknowledgements

We thank A. Miyazaki for providing strains, and S. Chaudhary and D. Perez de Camino for technical help. The Phycomyces genome sequence was provided by the US Department of Energy Joint Genome Institute (Office of Science of the Department of Energy, DE-AC02-05CH11231). This research was funded by the US National Science Foundation (MCB-0920581), by Spanish grants (BIO2009-12486, AGL2005-08081, J. Andalucia P08-CVI-03901, P09-CVI-5027 and CVI 910, J. Castilla-Leon GR64), which are supported by the European Regional Development Fund, and a loan from the Educafin Program, Mexico.