Journal article

Somaclonal breeding of reed canarygrass (Phalaris arundinacea L.)

G Gyulai, Z Mester, J Kiss, L Szeman, A Idnurm, L Heszky

GRASS AND FORAGE SCIENCE | BLACKWELL PUBLISHING LTD | Published : 2003

Abstract

Tissue culture-induced variants (somaclones) of reed canarygrass (Phalaris arundinacea L.) were developed from callus cells of aseptic spikelet cultures. Molecular polymorphism of the populations of somaclones and spikelet-donor plants as natural vegetative clones were compared by the application of polymerase chain reaction (PCR)-based techniques of random amplified polymorphic DNA (RAPD)-, simple sequence repeat (SSR)-, and inter-simple sequence repeat (ISSR)-PCR to genomic DNA samples. The genetic diversity was higher in the somaclones compared with vegetative clones as measured by the maximum genetic distance (MaxGD) which increased from 0.401 (vegetative clones) to 0.498 (somaclones). A..

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University of Melbourne Researchers