Two Origins for the Gene Encoding alpha-Isopropylmalate Synthase in Fungi
Erica M Larson, Alexander Idnurm
PLOS ONE | PUBLIC LIBRARY SCIENCE | Published : 2010
BACKGROUND: The biosynthesis of leucine is a biochemical pathway common to prokaryotes, plants and fungi, but absent from humans and animals. The pathway is a proposed target for antimicrobial therapy. METHODOLOGY/PRINCIPAL FINDINGS: Here we identified the leuA gene encoding alpha-isopropylmalate synthase in the zygomycete fungus Phycomyces blakesleeanus using a genetic mapping approach with crosses between wild type and leucine auxotrophic strains. To confirm the function of the gene, Phycomyces leuA was used to complement the auxotrophic phenotype exhibited by mutation of the leu3+ gene of the ascomycete fungus Schizosaccharomyces pombe. Phylogenetic analysis revealed that the leuA gene in..View full abstract
Awarded by US National Science Foundation
This research was supported by a University of Missouri-Kansas City (UMKC) Faculty Research Grant and US National Science Foundation grant MCB-0920581. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.