Journal article
Development of resources for the analysis of gene function in Pucciniomycotina red yeasts
G Ianiri, SAI Wright, R Castoria, A Idnurm
Fungal Genetics and Biology | ACADEMIC PRESS INC ELSEVIER SCIENCE | Published : 2011
Abstract
The Pucciniomycotina is an important subphylum of basidiomycete fungi but with limited tools to analyze gene functions. Transformation protocols were established for a Sporobolomyces species (strain IAM 13481), the first Pucciniomycotina species with a completed draft genome sequence, to enable assessment of gene function through phenotypic characterization of mutant strains. Transformation markers were the URA3 and URA5 genes that enable selection and counter-selection based on uracil auxotrophy and resistance to 5-fluoroorotic acid. The wild type copies of these genes were cloned into plasmids that were used for transformation of Sporobolomyces sp. by both biolistic and Agrobacterium-media..
View full abstractGrants
Awarded by U.S. Department of Energy
Funding Acknowledgements
We thank Geraldine Butler and Kenneth Wolfe for providing strain IAM 13481, and the Fungal Genetics Stock Center for housing and distributing the resources reported here. We gratefully acknowledge access to the genome sequence of Sporobolomyces sp. strain IAM 13481: the work conducted by the US Department of Energy Joint Genome Institute is supported by the Office of Science of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The present research was supported in part by the U.S. National Science Foundation (Grant MCB-0920581 to A.I.), by a U.S.-Italian Joint Commission on Scientific and Technological Cooperation grant (to R.C., S.A.I.W. and A.I.), by the Italian Ministry for University and Scientific Research (MIUR) within the frame of "Incentivazione alla mobilita di studiosi stranieri e italiani residenti all'estero" (DM 1.2.2005, n.18; to S.A.I.W.), by PRIN 2008 - prot. 2008JKH2MM - "Exploitation of genes and proteins from different biological sources for limiting patulin contamination caused by the pathogenic fungus Penicillium expansum" (to R.C.), and by a grant from the Dottorato di Ricerca in Difesa e Qualita delle Produzioni Agro-alimentari e Forestali (to G.I.).