Methylation profiling of ductal carcinoma in situ and its relationship to histopathological features
Jia-Min B Pang, Siddhartha Deb, Elena A Takano, David J Byrne, Nicholas Jene, Alice Boulghourjian, Anne Holliday, Ewan Millar, C Soon Lee, Sandra A O'Toole, Alexander Dobrovic, Stephen B Fox
Breast Cancer Research | BMC | Published : 2014
INTRODUCTION: DNA methylation is a well-studied biomarker in invasive breast cancer, but its role in ductal carcinoma in situ (DCIS) is less well characterized. The aims of this study are to assess the methylation profile in DCIS for a panel of well-characterized genes that are frequently methylated in breast cancer, to investigate the relationship of methylation with pathological features, and to perform a proof-of-principle study to evaluate the practicality of methylation as a biomarker in diagnostic DCIS material. METHODS: Promoter CpG island methylation for a panel of 11 breast cancer-related genes was performed by methylation-sensitive high resolution melting (MS-HRM). Formalin-fixed, ..View full abstract
Awarded by Department of Defense Breast Cancer Research Program
We thank Ida Candiloro and Hongdo Do for advice on primer design and for access to unpublished primer sequences, MS-HRM assay optimization and interpretation of MS-HRM data. This project is funded by grants to SBF and AD from Cancer Australia and the National Breast Cancer Foundation, Australia, to AD from the Cancer Council of Victoria and the Department of Defense Breast Cancer Research Program under award number W81XWH-051-0500, and to SOT from The Cancer Institute NSW, and the Sydney Breast Cancer Foundation.