Journal article
Dysregulation of Complement System and CD4 T Cell Activation Pathways Implicated in Allergic Response
A Couto Alves, S Bruhn, A Ramasamy, H Wang, JW Holloway, AL Hartikainen, MR Jarvelin, M Benson, DJ Balding, LJM Coin
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2013
Open access
Abstract
Allergy is a complex disease that is likely to involve dysregulated CD4+ T cell activation. Here we propose a novel methodology to gain insight into how coordinated behaviour emerges between disease-dysregulated pathways in response to pathophysiological stimuli. Using peripheral blood mononuclear cells of allergic rhinitis patients and controls cultured with and without pollen allergens, we integrate CD4+ T cell gene expression from microarray data and genetic markers of allergic sensitisation from GWAS data at the pathway level using enrichment analysis; implicating the complement system in both cellular and systemic response to pollen allergens. We delineate a novel disease network linkin..
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Awarded by European Commission
Funding Acknowledgements
ACA, LC, DB, SB, JH, and MB acknowledge funding from European Commission, Framework 7, grant number 223367 (MultiMod). NFBC1966 received financial support from the Academy of Finland (project grants 104781, 120315, 129269, 1114194, 139900/24300796, Centre of Excellence in Complex Disease Genetics and SALVE), University Hospital Oulu, Biocenter, University of Oulu (75617), NHLBI grant 5R01HL087679-02 through the STAMPEED program (1RL1MH083268-01), NIH/NIMH (5R01MH63706:02), ENGAGE project HEALTH-F4-2007-201413, the Medical Research Council, UK (PrevMetSyn/SALVE). The DNA extractions, sample quality controls, biobank upkeep and aliquotting was performed in the National Public Health Institute, Biomedicum Helsinki, Finland and supported financially by the Academy of Finland and Biocentrum Helsinki. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.