Journal article
Differential coexpression analysis of obesity-associated networks in human subcutaneous adipose tissue
AJ Walley, P Jacobson, M Falchi, L Bottolo, JC Andersson, E Petretto, A Bonnefond, E Vaillant, C Lecoeur, V Vatin, M Jernas, D Balding, M Petteni, YS Park, T Aitman, S Richardson, L Sjostrom, LMS Carlsson, P Froguel
International Journal of Obesity | Published : 2012
DOI: 10.1038/ijo.2011.22
Abstract
Objective: To use a unique obesity-discordant sib-pair study design to combine differential expression analysis, expression quantitative trait loci (eQTLs) mapping and a coexpression regulatory network approach in subcutaneous human adipose tissue to identify genes relevant to the obese state.Study design:Genome-wide transcript expression in subcutaneous human adipose tissue was measured using Affymetrix U133 Plus 2.0 microarrays (Affymetrix, Santa Clara, CA, USA), and genome-wide genotyping data was obtained using an Applied Biosystems (Applied Biosystems; Life Technologies, Carlsbad, CA, USA) SNPlex linkage panel. Subjects: A total of 154 Swedish families ascertained through an obese proba..
View full abstractGrants
Awarded by Diabetesförbundet
Funding Acknowledgements
We wish to acknowledge the participation of all the families and clinical staff involved in the SOS SibPair study. We thank Professor Eric Schadt for advice and the provision of the mouse data set and the staff of the Imperial College High-Performance Computing Service for their advice and support. This study was funded by Grant no. 079534/z/06/z from the Wellcome Trust, the Swedish Research Council (K2010-55X-11285-13), the Swedish foundation for Strategic Research to Sahlgrenska Center for Cardiovascular and Metabolic Research, the Swedish Diabetes foundation and the Swedish federal government under the LUA/ALF agreement. Sylvia Richardson acknowledges support from the MRC Grant G0600609.