Journal article

Whey Protein Ingestion Activates mTOR-dependent Signalling after Resistance Exercise in Young Men: A Double-Blinded Randomized Controlled Trial

Michelle M Farnfield, Kate A Carey, Petra Gran, Marissa K Trenerry, David Cameron-Smith

NUTRIENTS | MDPI | Published : 2009


The effect of resistance exercise with the ingestion of supplementary protein on the activation of the mTOR cascade, in human skeletal muscle has not been fully elucidated. In this study, the impact of a single bout of resistance exercise, immediately followed by a single dose of whey protein isolate (WPI) or placebo supplement, on the activation of mTOR signalling was analyzed. Young untrained men completed a maximal single-legged knee extension exercise bout and were randomized to ingest either WPI supplement (n = 7) or the placebo (n = 7). Muscle biopsies were taken from the vastus lateralis before, and 2, 4 and 24 h post-exercise. WPI or placebo ingestion consumed immediately post-exerci..

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University of Melbourne Researchers


Awarded by Dairy Australia

Funding Acknowledgements

The authors acknowledge the excellent medical assistance provided by Andrew Garnham, School of Exercise and Nutrition Sciences, Deakin University; Murray Goulburn Nutritionals for providing the supplements; Craige Trenerry and Vilnis Ezernieks from Department for Primary Industries, Werribee, Australia for the amino acid analysis of the supplements; Jane Tulloch and Jennifer White for their technical assistance. This research was supported by a grant awarded to D. Cameron-Smith from Dairy Australia (DU1111; Dairy Protein Supplementation to Increase Muscle Strength). <SUP>a</SUP> Supported by Dairy Australia. WPI provided as an in kind gift from Murray Goulburn Nutritionals, Australia.