Localization of type II phospholipase A2 messenger RNA and immunoactivity in human placenta and fetal membranes

Abstract

Although Type II phospholipase A2 (PLA2) immunoactivity has been identified in homogenates of human placenta and fetal membranes, there is a paucity of information concerning the sites of synthesis of this secreted PLA2, isozyme. The aim of this study, therefore, was to establish the cellular localization of Type II PLA2, messenger RNA (mRNA) in human term placental and fetal membranes by in situ hybridization. In addition, the co-localization of immunoreactive Type II PLA2, in gestational tissues was determined, and the effect of labour status and pre-eclampsia on immunolabelling intensity were established. Type II PLA2, mRNA was identified in all tissue sections examined and was localized ..