Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm
David AF Loebel, Joshua B Studdert, Melinda Power, Tania Radziewic, Vanessa Jones, Leigh Coultas, Yvette Jackson, Renuka S Rao, Kirsten Steiner, Nicolas Fossat, Lorraine Robb, Patrick PL Tam
Development | COMPANY OF BIOLOGISTS LTD | Published : 2011
Rhou encodes a Cdc42-related atypical Rho GTPase that influences actin organization in cultured cells. In mouse embryos at early-somite to early-organogenesis stages, Rhou is expressed in the columnar endoderm epithelium lining the lateral and ventral wall of the anterior intestinal portal. During foregut development, Rhou is downregulated in regions where the epithelium acquires a multilayered morphology heralding the budding of organ primordia. In embryos generated from Rhou knockdown embryonic stem (ES) cells, the embryonic foregut displays an abnormally flattened shape. The epithelial architecture of the endoderm is disrupted, the cells are depleted of microvilli and the phalloidin-stain..View full abstract
Awarded by NHMRC of Australia
Awarded by Australian Research Council
Awarded by NHMRC
This work was supported by the NHMRC of Australia (grant 323704), the Australian Research Council (grant DP0985052) and Mr James Fairfax. DAFL is a Kimberly Clark Research Fellow, N.F. is a University of Sydney Post-Doctoral Fellow and P. P. L. T. is a NHMRC Senior Principal Research Fellow (grant 372102).