Journal article

Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm

David AF Loebel, Joshua B Studdert, Melinda Power, Tania Radziewic, Vanessa Jones, Leigh Coultas, Yvette Jackson, Renuka S Rao, Kirsten Steiner, Nicolas Fossat, Lorraine Robb, Patrick PL Tam

Development | COMPANY OF BIOLOGISTS LTD | Published : 2011

Abstract

Rhou encodes a Cdc42-related atypical Rho GTPase that influences actin organization in cultured cells. In mouse embryos at early-somite to early-organogenesis stages, Rhou is expressed in the columnar endoderm epithelium lining the lateral and ventral wall of the anterior intestinal portal. During foregut development, Rhou is downregulated in regions where the epithelium acquires a multilayered morphology heralding the budding of organ primordia. In embryos generated from Rhou knockdown embryonic stem (ES) cells, the embryonic foregut displays an abnormally flattened shape. The epithelial architecture of the endoderm is disrupted, the cells are depleted of microvilli and the phalloidin-stain..

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University of Melbourne Researchers