Journal article

DMRT1 is required for Mullerian duct formation in the chicken embryo

KL Ayers, AD Cutting, KN Roeszler, AH Sinclair, CA Smith



DMRT1 is a conserved transcription factor with a central role in gonadal sex differentiation. In all vertebrates studied, DMRT1 plays an essential function in testis development and/or maintenance. No studies have reported a role for DMRT1 outside the gonads. Here, we show that DMRT1 is expressed in the paired Müllerian ducts in the chicken embryo, where it is required for duct formation. DMRT1 mRNA and protein are expressed in the early forming Müllerian ridge, and in cells undergoing an epithelial to mesenchyme transition during duct morphogenesis. RNAi-mediated knockdown of DMRT1 in ovo causes a greatly reduced mesenchymal layer, which blocks caudal extension of the duct luminal epitheliu..

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Awarded by Australian Research Council (ARC) Future Fellowship

Awarded by Australian Government's Cooperative Research Centres Program (Poultry CRC)

Funding Acknowledgements

The authors thank Dr. Olivier Serralbo (Australian Regenerative Medicine Institute, Melbourne) for demonstrating the in ovo electroporation technique. CAS is funded through an Australian Research Council (ARC) Future Fellowship (Grant number FT100100750). This research was conducted with support from the Poultry CRC, established and supported under the Australian Government's Cooperative Research Centres Program (Poultry CRC, grant number 1.01). The Murdoch Childrens Research Institute (MCRI) is supported by the Victorian Government's Operational Infrastructure Support Program.