Journal article

Physicochemical and Immunological Assessment of Engineered Pure Protein Particles with Different Redox States

Katelyn T Gause, Yan Yan, Jiwei Cui, Neil M O'Brien-Simpson, Jason C Lenzo, Eric C Reynolds, Frank Caruso

ACS NANO | AMER CHEMICAL SOC | Published : 2015

Abstract

The development of subunit antigen delivery formulations has become an important research endeavor, especially in cases where a whole cell vaccine approach has significant biosafety issues. Particle-based systems have shown particular efficacy due to their inherent immunogenicity. In some cases, fabrication techniques can lead to changes in the redox states of encapsulated protein antigens. By employing a uniform, well-characterized, single-protein system, it is possible to elucidate how the molecular details of particle-based protein antigens affect their induced immune responses. Using mesoporous silica-templated, amide bond-stabilized ovalbumin particles, three types of particles were fab..

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Grants

Awarded by Australian Research Council


Awarded by Discovery Early Career Researcher Award


Awarded by ARC Centre of Excellence


Awarded by National Health and Medical Research Council


Funding Acknowledgements

This work was supported by the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030), Discovery Early Career Researcher Award (Y.Y., DE130100488), and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology (CE140100036) schemes, as well as the National Health and Medical Research Council Project Grant (APP1029878). K.T.G. acknowledges funding from the Australian Government through an International Postgraduate Research Scholarship and an Australian Postgraduate Award.