Journal article

Monoclonal Antibody-Functionalized Multilayered Particles: Targeting Cancer Cells in the Presence of Protein Coronas

Qiong Dai, Yan Yan, Ching-Seng Ang, Kristian Kempe, Marloes MJ Kamphuis, Sarah J Dodds, Frank Caruso

ACS NANO | AMER CHEMICAL SOC | Published : 2015

Abstract

Engineered particles adsorb biomolecules (e.g., proteins) when introduced in a biological medium to form a layer called a "corona". Coronas, in particular the protein corona, play an important role in determining the surface properties of particles and their targeting abilities. This study examines the influence of protein coronas on the targeting ability of layer-by-layer (LbL)-assembled polymer capsules and core-shell particles functionalized with monoclonal antibodies. Upon exposure of humanized A33 monoclonal antibody (huA33 mAb)-functionalized poly(methacrylic acid) (PMA) capsules or huA33 mAb-PMA particles to human serum, a total of 83 or 65 proteins were identified in the protein coro..

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Grants

Awarded by Australian Research Council under the Australian Laureate Fellowship


Awarded by Discovery Early Career Researcher Award


Awarded by Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology


Funding Acknowledgements

This work was supported by the Australian Research Council under the Australian Laureate Fellowship (F.C., FL120100030), Discovery Early Career Researcher Award (Y.Y., DE130100488), and the Australian Research Council Centre of Excellence in Convergent Bio-Nano Science and Technology (project number CE140100036) schemes, as well as the Feodor-Lynen Fellowship from the Alexander von Humboldt Foundation (K.K.). Q.D. acknowledges funding from the Australian Government through an International Postgraduate Research Scholarship and an Australian Postgraduate Award. We acknowledge Ms. Marta Redrado Notivoli (platform support officer colloidal characterization from The Melbourne Materials Institute) for assistance with acquisition of AFM images, Dr. Huanli Sun for assistance with NMR measurement and analysis, and Mr. Junling Guo for assistance with acquisition of TEM images.