Diurnal variation and short-term pre-analytical stability of serum soluble α-klotho in healthy volunteers: a pilot study

Abstract

Aim To investigate the diurnal variability and pre-analytical stability of soluble α-klotho in serum. Background Recent evidence suggests that the cleaved extracellular domain of the α-klotho receptor, soluble α-klotho, affects phosphate homeostasis, ion channel regulation and antifibrotic/antioxidant pathways. However, soluble α-klotho measurements have yielded inconsistent results when related to renal function, markers of mineral metabolism and patient outcomes. Pre-analytical factors such as biological variation and analyte stability may affect the interpretation of soluble α-klotho results but are yet to be formally assessed. Methods Clotted blood samples were collected from 10 healthy ..