Journal article
Relative abundance of fetuin- A in peritoneal dialysis effluent and its association with in situ formation of calciprotein particles: An observational pilot study
MMX Cai, B Wigg, ER Smith, TD Hewitson, LP McMahon, SG Holt
Nephrology | WILEY | Published : 2015
DOI: 10.1111/nep.12350
Abstract
Aim In patients with renal failure or chronic inflammation, the accumulation of fetuin-A-containing calciprotein particles (CPP) in the extracellular fluid has been implicated in driving inflammatory pathways and extraosseous mineral deposition. We aimed to discover whether CPP are present in the peritoneal dialysis fluid effluent (PDF) of stable peritoneal dialysis (PD) patients, and if so, how these particles might be formed.Methods Serum and PDF were sampled from 20 stable PD patients. CPP were quantified by the reduction in fetuin-A concentration after high speed centrifugation. 8-iso-PGF2α in PDF was measured as a marker of oxidative stress. Fetuin-A and phosphate were added to commerci..
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Funding Acknowledgements
The authors were supported by an unrestricted investigator initiated grant from Amgen Australia Pty Ltd. SGH is the recipient of a Jacquot Research Fellowship, whereas MC is supported by an NHMRC biomedical scholarship.