Journal article

Pirfenidone reduces in vitro rat renal fibroblast activation and mitogenesis

TD Hewitson, KJ Kelynack, MG Tait, M Martic, CL Jones, SB Margolin, GJ Becker

JOURNAL OF NEPHROLOGY | SPRINGER HEIDELBERG | Published : 2001

Abstract

BACKGROUND: Fibroblasts have been universally recognised in tubulointerstitial injury, where their presence has been shown to be a marker of disease progression. Recently, pirfenidone (PF) has been shown to both ameliorate progressive fibrosis and reduce established scarring after ureteric obstruction (UUO) in the rat, suggesting that it is a novel anti-fibrotic agent. The objective of this study was therefore to determine if these effects include down-regulation of fibroblast function. METHODS: Cortical fibroblasts were obtained from outgrowth cultures of renal tissue isolated from kidneys 3 days after UUO and constituted 100% of cells studied. Functional studies examined the effects of 20 ..

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