Journal article

CHARACTERIZATION OF IMMOBILIZED ESCHERICHIA-COLI ALKALINE-PHOSPHATASE REACTORS IN FLOW-INJECTION ANALYSIS

Y SHAN, ID MCKELVIE, BT HART

ANALYTICAL CHEMISTRY | AMER CHEMICAL SOC | Published : 1993

Abstract

The characterization of immobilized Escherichia coli alkaline phosphatase reactors used in flow injection analysis is reported for factors such as optimum pH, activity, ionic strength, product inhibition, and substrate specificity. The kinetics of the immobilized enzyme was studied, and mathematical descriptions were developed for the use of an immobilized enzyme packed-bed reactor to evaluate the kinetic parameters and the number of active sites on the immobilized enzyme. Suppression of phosphatase activity by orthophosphate was found to be significantly reduced, and the Michaelis-Menten constant increased when the enzyme was immobilized and packed in a reactor. Immobilized E. coli alkaline..

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