Journal article
Non-protein amino acids in Australian acacia seed: Implications for food security and recommended processing methods to reduce djenkolic acid
BA Boughton, P Reddy, MP Boland, U Roessner, P Yates
Food Chemistry | ELSEVIER SCI LTD | Published : 2015
Abstract
Seed of Australian acacia species, Acacia colei, Acacia elecantha, Acacia torulosa, Acacia turmida and Acacia saligna, were analysed for the presence of toxic non-protein amino acids and the levels of essential amino acids. Amines were derivatised with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate before analysis using liquid chromatography electrospray ionisation triple quadrupole mass spectrometry (LC-ESI-QQQ-MS). Multiple reaction monitoring (MRM) with optimised transitions and collision energies for each analyte were employed. The known nephrotoxic compound djenkolic acid was found to be present at elevated levels in all species tested. The lowest levels were in A. colei (0.49% w/w) an..
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Funding Acknowledgements
[ "The authors wish to acknowledge Ms. Nirupama Jayasinghe, Ms. Alice Ng and Ms Terra Stark, Metabolomics Australia, for expert laboratory assistance. The authors also thank the School of Botany node of Metabolomics Australia (MA) at The University of Melbourne, a member of Bioplatforms Australia Pty. Ltd. which is funded through the National Collaborative Research Infrastructure Strategy (NCRIS), 5.1 Biomolecular Platforms and Informatics and co-investments from the Victorian Government.", "Funding for the research was provided by World Vision Australia." ]