Journal article
Diverse and heritable lineage imprinting of early haematopoietic progenitors
SH Naik, L Perié, E Swart, C Gerlach, N Van Rooij, RJ De Boer, TN Schumacher
Nature | NATURE PUBLISHING GROUP | Published : 2013
DOI: 10.1038/nature12013
Abstract
Haematopoietic stem cells (HSCs) and their subsequent progenitors produce blood cells, but the precise nature and kinetics of this production is a contentious issue. In one model, lymphoid and myeloid production branch after the lymphoid-primed multipotent progenitor (LMPP), with both branches subsequently producing dendritic cells. However, this model is based mainly on in vitro clonal assays and population-based tracking in vivo, which could miss in vivo single-cell complexity. Here we avoid these issues by using a new quantitative version of 'cellular barcoding' to trace the in vivo fate of hundreds of LMPPs and HSCs at the single-cell level. These data demonstrate that LMPPs are highly h..
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Awarded by Seventh Framework Programme
Funding Acknowledgements
We thank G. Filion, U. Braunschweig, L. Pagie, M. Hauptmann, P. Lio, R. van der Wath and the NKI Genomics Facility for computational assistance; J. van Heijst, J. Rohr and J. Urbanus for useful discussions; http://www.josharris.com for illustrations in Fig. 1a and Supplementary Fig. 1; NKI cytometry and animal facilities; D. Leone, Z. Ping and M. Lodder for technical assistance; and P. Hodgkin, K. Duffy and J. Coquet for critical reading of the manuscript. This work was supported by ERC grant LIFE-HIS-T and HFSP grant RGP0060/2012, S.H.N. was supported by the National Health and Medical Research Council Australia, Marie Curie Incoming International FP6 Fellowship, and the Leukemia and Lymphoma Society; and L. P. was supported by a Marie Curie Intra European FP7 Fellowship and the Bettencourt Schueller Fondation.