Journal article

Diverse and heritable lineage imprinting of early haematopoietic progenitors

Shalin H Naik, Leila Perie, Erwin Swart, Carmen Gerlach, Nienke van Rooij, Rob J de Boer, Ton N Schumacher

Nature | NATURE PUBLISHING GROUP | Published : 2013

Abstract

Haematopoietic stem cells (HSCs) and their subsequent progenitors produce blood cells, but the precise nature and kinetics of this production is a contentious issue. In one model, lymphoid and myeloid production branch after the lymphoid-primed multipotent progenitor (LMPP), with both branches subsequently producing dendritic cells. However, this model is based mainly on in vitro clonal assays and population-based tracking in vivo, which could miss in vivo single-cell complexity. Here we avoid these issues by using a new quantitative version of 'cellular barcoding' to trace the in vivo fate of hundreds of LMPPs and HSCs at the single-cell level. These data demonstrate that LMPPs are highly h..

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University of Melbourne Researchers

Grants

Awarded by ERC grant LIFE-HIS-T and HFSP


Funding Acknowledgements

We thank G. Filion, U. Braunschweig, L. Pagie, M. Hauptmann, P. Lio, R. van der Wath and the NKI Genomics Facility for computational assistance; J. van Heijst, J. Rohr and J. Urbanus for useful discussions; http://www.josharris.com for illustrations in Fig. 1a and Supplementary Fig. 1; NKI cytometry and animal facilities; D. Leone, Z. Ping and M. Lodder for technical assistance; and P. Hodgkin, K. Duffy and J. Coquet for critical reading of the manuscript. This work was supported by ERC grant LIFE-HIS-T and HFSP grant RGP0060/2012, S.H.N. was supported by the National Health and Medical Research Council Australia, Marie Curie Incoming International FP6 Fellowship, and the Leukemia and Lymphoma Society; and L. P. was supported by a Marie Curie Intra European FP7 Fellowship and the Bettencourt Schueller Fondation.