Journal article
Cytokine profile and induction of T helper type 17 and regulatory T cells by human peripheral mononuclear cells after microbial exposure
ON Donkor, M Ravikumar, O Proudfoot, SL Day, V Apostolopoulos, G Paukovics, T Vasiljevic, SL Nutt, H Gill
Clinical and Experimental Immunology | Published : 2012
Abstract
The immunomodulatory effects of probiotics were assessed following exposure of normal peripheral blood mononuclear cells (PBMC), cord blood cells and the spleen-derived monocyte/macrophage cell line CRL-9850 to Lactobacillus acidophilus LAVRI-A1, Lb. rhamnosus GG, exopolysaccharides (EPS)-producing Streptococcus thermophilus St1275, Bifidobacteriun longum BL536, B. lactis B94 and Escherichia coli TG1 strains. The production of a panel of pro- and anti-inflammatory cytokines by PBMC following bacterial stimulation was measured, using live, heat-killed or mock gastrointestinal tract (GIT)-exposed bacteria, and results show that (i) all bacterial strains investigated induced significant secreti..
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Funding Acknowledgements
This research was funded by the Victoria University Research Fellow Grant and the Researcher Development Grants Scheme. We thank the Australian Red Cross Services and the Cord Blood Bank (BMDI, Royal Children Hospital, Melbourne, Australia) for their supply of blood products. The in-kind financial and technical supports by Burnet Institute, and The Walter and Eliza Hall Institute of Medical Research (Parkville, VIC, Australia) are also gratefully acknowledged. Researches at the Walter and Eliza Hall and Burnet Institutes were made possible through Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS.