Journal article
M-CSF instructs myeloid lineage fate in single haematopoietic stem cells
N Mossadegh-Keller, S Sarrazin, PK Kandalla, L Espinosa, E Richard Stanley, SL Nutt, J Moore, MH Sieweke
Nature | Published : 2013
DOI: 10.1038/nature12026
Abstract
Under stress conditions such as infection or inflammation the body rapidly needs to generate new blood cells that are adapted to the challenge. Haematopoietic cytokines are known to increase output of specific mature cells by affecting survival, expansion and differentiation of lineage-committed progenitors, but it has been debated whether long-term haematopoietic stem cells (HSCs) are susceptible to direct lineage-specifying effects of cytokines. Although genetic changes in transcription factor balance can sensitize HSCs to cytokine instruction, the initiation of HSC commitment is generally thought to be triggered by stochastic fluctuation in cell-intrinsic regulators such as lineage-specif..
View full abstractGrants
Awarded by National Institutes of Health
Funding Acknowledgements
We acknowledge grants from the 'Association pour la recherche sur le Cancer' (3422) and the 'Agence nationale de la Recherche' (BLAN07-1_205752). We thank P. Kastner and S. Chan for PU.1-GFP reporter mice; T. P. VuManh and J. Maurizio for bioinformatics; M. Barad, A. Zouine and M.-L. Thibult for flow cytometry support; L. Razafindramanana for animal handling; and J. Favret, P. Perrin and L. Chasson for tissue sectioning. E. R. S. is supported by NIH grant CA 32551. S.L.N. is an Australian Research Council Future Fellow and received Victorian State Government Operational and Australian Government NHMRC Independent Research Institute Infrastructure Support. M. H. S. is a 'Fondation pour la Recherche Medicale' (DEQ20071210559; DEQ20110421320) and INSERM-Helmholtz group leader.