INTERACTIONS BETWEEN REFLEXES EVOKED BY DISTENSION AND MUCOSAL STIMULATION - ELECTROPHYSIOLOGICAL STUDIES OF GUINEA-PIG ILEUM

Abstract

Intracellular recording methods were used to examine stereotyped reflexes evoked in the circular muscle of guinea-pig small intestine by distension or repetitive deformation of the mucosal villi, in vitro. Both stimuli evoked compound excitatory junction potentials (EJPs) on the oral side of the site of stimulation and compound inhibitory junction potentials (IJPs) on the anal side. Stimulation of the mucosa by application of 10 microliters of 0.5 M HCl evoked similar reflex responses in the circular muscle. The compound EJPs evoked by mucosal stimulation were depressed, but not abolished, by 1 microM hyoscine, indicating that these responses were partially mediated by release of acetylcholi..