Journal article

TOTAL NUMBERS OF NEURONS IN MYENTERIC GANGLIA OF THE GUINEA-PIG SMALL-INTESTINE

HM YOUNG, JB FURNESS, P SEWELL, EF BURCHER, CJ KANDIAH

CELL AND TISSUE RESEARCH | SPRINGER VERLAG | Published : 1993

Abstract

Two techniques that are thought to stain all of the neurons in the myenteric ganglia of the intestine are NADH diaphorase histochemistry and immunohistochemistry using a "nerve cell body" antiserum. However, this assumption has never been directly verified. In the present study myenteric ganglia of the guinea-pig ileum were prepared as whole-mounts and stained with either of these techniques. All nerve cells that could be identified in the whole-mounts were counted. The whole-mounts were then embedded flat in resin and serially sectioned at 1 micron. Nerve cells were identified and counted from the serial sections, and the data compared to those obtained from the whole-mounts. NADH diaphoras..

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