Journal article

SIMULTANEOUS FIXATION AND PRODUCTION OF CATECHOLAMINE FLUORESCENCE IN CENTRAL NERVOUS-TISSUE BY PERFUSION WITH ALDEHYDES

JB FURNESS, M COSTA, WW BLESSING

HISTOCHEMICAL JOURNAL | CHAPMAN HALL LTD | Published : 1977

Abstract

Perfusion with a mixture of formaldehyde (4%) and glutaraldehyde (0.5%) is shown both to fix central nervous tissue and to produce, with no further treatment, a fluorescence histochemical localization of catecholamines. After perfusion, serial sections can be readily taken through the whole brain with a Vibratome. Landmarks which are apparent at low power with white-light illumination can be seen when the sections are viewed in the fluorescence microscope. Catecholamine-containing nerve cell bodies, varicose axon terminals and non-varicose nerve fibres appear brightly fluorescent and well localized. The method has been applied to rats, guinea-pigs and rabbits and is ideally suited to the acc..

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University of Melbourne Researchers