Journal article

USE OF GLYOXYLIC-ACID FOR FLUORESCENCE HISTOCHEMICAL DEMONSTRATION OF PERIPHERAL STORES OF NORADRENALINE AND 5-HYDROXYTRYPTAMINE IN WHOLE MOUNTS

JB FURNESS, M COSTA

HISTOCHEMISTRY | SPRINGER | Published : 1975

Abstract

The reactions of glyoxylic acid with peripheral stores of noradrenaline and 5-hydroxytryptamine to provide a fluorescence histochemical method for their localization have been investigated. Incubation in glyoxylic acid, followed by drying and heating of whole mount preparations gives an intense and well localized reaction. For incubation, a concentration of 2% glyoxylic acid, buffered to pH 7 at room temperature for 30 minutes gives ideal results. The method is equally good if the pH is varied in the range 6 to 9 or if the tissue is stored in the incubation mixture for up to 6 hours. Ideal development of the fluorophore requires an initial excess of moisture in the tissue, that this moisture..

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University of Melbourne Researchers