Journal article

PRESENCE OF AROMATIC L-AMINO-ACID DECARBOXYLASE IN CERTAIN INTESTINAL NERVE-CELLS

M COSTA, JB FURNESS, JR MCLEAN

HISTOCHEMISTRY | SPRINGER VERLAG | Published : 1976

Abstract

The presence of aromatic 1-amino acid decarboxylase (AADC) in nerve cell bodies of the intrinsic plexuses of the guinea-pig small intestine was demonstrated by incubating segments of intestine with 1-dopa in the presence of an inhibitor of monoamine oxidase, pargyline. After such incubation, some nerve cell bodies gave a fluorescence histochemical reaction indicative of the presence of a decarboxylated product of 1-dopa, probably dopamine. No fluorescence reaction occurred in the unincubated control or if the inhibitor of AADC, RO 4-4602, was included in the incubation mixture. The AADC-containing cell bodies apparently do not take up and store dopamine, because no fluorescence could be dete..

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University of Melbourne Researchers