Journal article

ASPECTS OF ARRANGEMENT OF ADRENERGIC INNERVATION IN GUINEA PIGS AS REVEALED BY FLUORESCENCE HISTOCHEMICAL METHOD APPLIED TO STRETCHED, AIR-DRIED PREPARATIONS

JB FURNESS, T MALMFORS

HISTOCHEMIE | Published : 1971

Abstract

The suitability of stretched, air-dried preparations for the fluorescence histochemical localization of catecholamines has been examined for a range of organs, including atrium, arteries, veins, digestive tract, urinogenital organs and sympathetic ganglia of the guinea-pig. Where possible, the tissue is divided into thin sheets under a dissecting microscope. It is then stretched over a glass slide to which it adheres as it dries. Stretch preparations can be easily and quickly prepared for the fluorescence localization of catecholamines and give clear and consistent results with many adrenergically innervated tissues. This technique allows the branching of adrenergic nerve trunks and of indiv..

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University of Melbourne Researchers