ISOLATION AND MICROSEQUENCE ANALYSIS OF A NOVEL FORM OF NEUROMEDIN-U FROM GUINEA-PIG SMALL-INTESTINE
R MURPHY, CA TURNER, JB FURNESS, L PARKER, A GIRAUD
PEPTIDES | PERGAMON-ELSEVIER SCIENCE LTD | Published : 1990
A multidimensional chromatographic regimen has been used to isolate and purify a peptide showing immunoreactivity for neuromedin U from guinea pig small intestine. Microsequence Edman N-terminal analysis and C-terminal analysis by enzymatic digestion showed this peptide to be a nonapeptide with the following sequence: H-Gly-Tyr-Phe-Leu-Phe-Arg-Pro-Arg-Asn-NH2. The C-terminal octapeptide of this sequence is the same as porcine NMU-8, and the C-terminal heptapeptide is identical to rat NMU(17-23).