Disruption of the Plasmodium falciparum liver-stage antigen-1 locus causes a differentiation defect in late liver-stage parasites
Sebastian A Mikolajczak, John B Sacci, Patricia De La Vega, Nelly Camargo, Kelly VanBuskirk, Urszula Krzych, Jun Cao, Marcelo Jacobs-Lorena, Alan F Cowman, Stefan HI Kappe
CELLULAR MICROBIOLOGY | WILEY | Published : 2011
The malaria parasite Plasmodium falciparum infects humans and first targets the liver where liver-stage parasites undergo pre-erythrocytic replication. Liver-stage antigen-1 (LSA-1) is currently the only identified P. falciparum protein for which expression is restricted to liver stages. Yet, the importance of LSA-1 for liver-stage parasite development remains unknown. Here we deleted LSA-1 in the NF54 strain of P. falciparum and analysed the lsa-1(-) parasites throughout their life cycle. lsa-1(-) sporozoites had normal gliding motility and invasion into hepatocytes. Six days after infection of a hepatocytic cell line, lsa-1(-) parasites exhibited a moderate phenotype with an ~50% reduction..View full abstract
Awarded by NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES
This work was supported by the Bill and Melinda Gates Foundation through the Foundation at the National Institutes of Health Grand Challenges in Global Health Initiative and the US Department of Defense.