Journal article

Use of multiplexed tandem PCR to estimate the prevalence and intensity of Theileria orientalis infections in cattle

Piyumali K Perera, Robin B Gasser, Elizabeth Read, Jakob Malmo, Nguyen Hanh, Simon Nyein, Allan Cheng, Aaron R Jex, Grant Rawlin, Terence W Spithill, Abdul Jabbar



This study employed a semi-quantitative, multiplexed tandem PCR (MT-PCR) to assess the prevalence and infection intensity of four genotypes (buffeli, chitose, ikeda and type 5) of Theileria orientalis in cattle in Australia. Genomic DNA samples from blood samples (n=448) collected from 27 to 32 dairy cows from each of 15 dairy herds with a history of recent theileriosis outbreaks (Group 1), and from blood samples available from 24 cows with or without oriental theileriosis (Group 2) were tested using MT-PCR. Results revealed that all four genotypes were present in Group 1 cattle; genotype buffeli had the highest prevalence (80.5%), followed by genotypes ikeda (71.4%), chitose (38.6%) and typ..

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Funding Acknowledgements

This project was partially supported by the Department of Agriculture Fisheries and Forestry (DAFF), the Victorian Cattle Compensation Fund, the Department of Environment and Primary Industries of Victoria, a Collaborative Research Grant (the University of Melbourne) (A.Ja.) and the Australian Research Council (ARC) (R.B.G. et al.). Funding from the CASS Foundation and The Ian Potter Foundation is also gratefully acknowledged (A.Ja.). P.P. is a grateful recipient of the International Postgraduate Research Scholarship (IPRS) and Australian Postgraduate Award (APA) through The University of Melbourne. We thank all the veterinary practitioners and dairy farmers who assisted by collecting blood samples.