Opposing roles for JNK and Aurora A in regulating the association of WDR62 with spindle microtubules
Nicholas R Lim, Yvonne YC Yeap, Teresa T Zhao, Yan Y Yip, Shu C Wong, Dan Xu, Ching-Seng Ang, Nicholas A Williamson, Zhiheng Xu, Marie A Bogoyevitch, Dominic CH Ng
JOURNAL OF CELL SCIENCE | COMPANY BIOLOGISTS LTD | Published : 2015
WD40-repeat protein 62 (WDR62) is a spindle pole protein required for normal cell division and neuroprogenitor differentiation during brain development. Microcephaly-associated mutations in WDR62 lead to mitotic mislocalization, highlighting a crucial requirement for precise WDR62 spatiotemporal distribution, although the regulatory mechanisms are unknown. Here, we demonstrate that the WD40-repeat region of WDR62 is required for microtubule association, whereas the disordered C-terminal region regulates cell-cycle-dependent compartmentalization. In agreement with a functional requirement for the WDR62–JNK1 complex during neurogenesis, WDR62 specifically recruits JNK1 (also known as MAPK8), b..View full abstract
Awarded by Australian Research Council Future Fellowship
Awarded by National Health and Medical Research Council Project Grant
D.C.H.N. is a recipient of an Australian Research Council Future Fellowship [grant number FT120100193]; and this work was supported by a National Health and Medical Research Council Project Grant [grant number APP1046032].