Journal article

Time-resolved confocal fluorescence microscopy of porphyrins for phototherapy

PG Spizzirri, JS Hill, SB Kahl, KP Ghiggino

LASERS IN MEDICAL SCIENCE | SPRINGER LONDON LTD | Published : 1996

Abstract

The application of a novel time-resolved confocal fluorescence microspectrometer to studies of the distribution and speciation of porphyrin photosensitizers in rat C6 cerebral glioma cells is described. The instrument combines a mode-locked argon ion laser excitation source with time-correlated single photon counting fluorescence detection and has sub-micron spatial and sub-nanosecond temporal resolution. The porphyrins studied were haematoporphyrin derivative (HpD), haematoporphyrin IX (HP), porphyrin c (Pc) and the tetrakiscarborane carboxylate ester of 2,4-(α,β-dihydroxyethyl) deuteroporphyrin IX (BOPP). From the heterogeneous emission observed in vitro, assignments and spatial location o..

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University of Melbourne Researchers