Journal article
Development and Validation of TaqMan Real-Time Polymerase Chain Reaction Assays for the Quantitative and Differential Detection of Wild-Type Infectious Laryngotracheitis Viruses from a Glycoprotein G–Deficient Candidate Vaccine Strain
Niraj K Shil, Alistair R Legione, Phillip F Markham, Amir H Noormohammadi, Joanne M Devlin
Avian Diseases | American Association of Avian Pathologists | Published : 2015
Abstract
Infectious laryngotracheitis (ILT) is a significant upper respiratory tract disease of chickens with a worldwide distribution. Differentiating between wild-type and vaccine strains of ILT virus (ILTV) would be useful for enhancing disease control, and in the early stages of a disease outbreak molecular diagnostic tools for the detection and differentiation of the circulating virus could be applied. This study developed TaqMan real-time PCR (qPCR) assays to detect and differentiate the glycoprotein G (gG)-deficient (ΔgG) ILTV candidate vaccine strain of ILTV from ILTV strains that contain the gG gene. The gG+ve and gG-ve ILTV TaqMan assays were used in individual and multiplex format to detec..
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Funding Acknowledgements
Experimental and laboratory samples used in this study were kindly provided by Sang-Won Lee and Mauricio J. C. Coppo, Faculty of Veterinary Science, The University of Melbourne, Australia. This research was partly conducted within the Poultry CRC, established and supported under the Australian Government's Research Centres Program.