Short communication: Bovine-derived proteins activate STAT3 in human skeletal muscle in vitro
MK Caldow, MR Digby, D Cameron-Smith
JOURNAL OF DAIRY SCIENCE | ELSEVIER SCIENCE INC | Published : 2015
Bovine milk contains biologically active peptides that may modulate growth and development within humans. In this study, targeted bovine-derived proteins were evaluated for their effects on signal transducer and activator of transcription-3 (STAT3) phosphorylation in human skeletal muscle cells. Following an acute exposure, bovine-derived acidic fibroblast growth factor-1 (FGF) and leukemia inhibitory factor (LIF) activated STAT3 in differentiating myotubes. Chronic exposure to FGF and LIF during the proliferative phase reduced myoblast proliferation and elevated MyoD and creatine kinase (CKM) mRNA expression without altering apoptotic genes. In mature myotubes, neither FGF nor LIF elicited ..View full abstract
Awarded by Dairy Australia
The authors thank Rani Watts (School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Australia) for her assistance with the collection of cell culture samples following the peptide initial screen and Andrew Garnham (School of Exercise and Nutrition Sciences, Deakin University, Melbourne, Australia) for performing the muscle biopsies. This research was funded by Dairy Australia (#DU12042). M. K. Caldow received a PhD scholarship from Dairy Australia; however, the funding body had no input in study design; in the collection, analysis, and interpretation of data; or in the writing of the report.