GM-CSF and uPA are required for Porphyromonas gingivalis-induced alveolar bone loss in a mouse periodontitis model

RS Lam; NM O'Brien-Simpson; JA Hamilton; JC Lenzo; JA Holden; GC Brammar; RK Orth; Y Tan; KA Walsh; AJ Fleetwood; EC Reynolds

Journal article

GM-CSF and uPA are required for Porphyromonas gingivalis-induced alveolar bone loss in a mouse periodontitis model

RS Lam, NM O'Brien-Simpson, JA Hamilton, JC Lenzo, JA Holden, GC Brammar, RK Orth, Y Tan, KA Walsh, AJ Fleetwood, EC Reynolds

Immunology and Cell Biology | NATURE PUBLISHING GROUP | Published : 2015

DOI: 10.1038/icb.2015.25

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Abstract

Granulocyte-macrophage colony-stimulating factor (GM-CSF) and urokinase-type plasminogen activator (uPA) can contribute to the progression of chronic inflammatory diseases with possible involvement of macrophages. In this study, we investigated the role of both GM-CSF and uPA in Porphyromonas gingivalis-induced experimental periodontitis using GM-CSF-/-and uPA-/-mice. Intra-oral inoculation of wild-type (WT) C57BL/6 mice with P. gingivalis resulted in establishment of the pathogen in plaque and a significant increase in alveolar bone resorption. The infected mice also exhibited a CD11b + CD86 + macrophage infiltrate into the gingival tissue, as well as P. gingivalis-specific pro-inflammatory..

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University of Melbourne Researchers

Neil O'Brien-Simpson Author

John Hamilton Author

James Holden Author

Yan Tan Author

Grants

Funding Acknowledgements

We thank Professor Stephen Kent and Dr Rob de Rose (Department of Microbiology and Immunology, The University of Melbourne) for the use of the AID ELISPOT plate reader. Ms Jenny Davis, Ms Rebecca Bowyer and Mr Christian Jarvinen are gratefully acknowledged for their assistance with the maintenance of the Biological Research Facility. This work was supported by the Oral Health Cooperative Research Centre, Australia.