Journal article

THYROID EPITHELIAL MORPHOGENESIS IN-VITRO - A ROLE FOR BUMETANIDE-SENSITIVE CL- SECRETION DURING FOLLICULAR LUMEN DEVELOPMENT

AS YAP, BR STEVENSON, JW ARMSTRONG, JR KEAST, SW MANLEY

EXPERIMENTAL CELL RESEARCH | ACADEMIC PRESS INC JNL-COMP SUBSCRIPTIONS | Published : 1994

Abstract

The structural and functional unit of the thyroid gland is the follicle, consisting of a closed lumen surrounded by a single layer of polarized epithelial cells. In this paper we have attempted to characterize the process of lumenal development when primary cultures of porcine thyroid cells reorganized to form follicles. Cells incubated with the loop diuretic, bumetanide, an inhibitor of NaK2Cl cotransport, aggregated but failed to form normal follicles. Laser scanning confocal microscopy combined with immunohistochemical markers of thyroid cell-surface proteins demonstrated that in the presence of bumetanide cells polarized and assembled ZO-1-containing tight junctions separating their apic..

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University of Melbourne Researchers