Glucose uptake during contraction in isolated skeletal muscles from neuronal nitric oxide synthase mu knockout mice
Yet Hoi Hong, Tony Frugier, Xinmei Zhang, Robyn M Murphy, Gordon S Lynch, Andrew C Betik, Stephen Rattigan, Glenn K McConell
JOURNAL OF APPLIED PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2015
Inhibition of nitric oxide synthase (NOS) significantly attenuates the increase in skeletal muscle glucose uptake during contraction/exercise, and a greater attenuation is observed in individuals with Type 2 diabetes compared with healthy individuals. Therefore, NO appears to play an important role in mediating muscle glucose uptake during contraction. In this study, we investigated the involvement of neuronal NOSμ (nNOSμ), the main NOS isoform activated during contraction, on skeletal muscle glucose uptake during ex vivo contraction. Extensor digitorum longus muscles were isolated from nNOSμ(-/-) and nNOSμ(+/+) mice. Muscles were contracted ex vivo in a temperature-controlled (30°C) organ b..View full abstract
The authors acknowledge funding from the National Health and Medical Research Council (NHMRC) of Australia and the Diabetes Australia Research Trust for the support for this study.