Journal article

Glucose uptake during contraction in isolated skeletal muscles from neuronal nitric oxide synthase mu knockout mice

Yet Hoi Hong, Tony Frugier, Xinmei Zhang, Robyn M Murphy, Gordon S Lynch, Andrew C Betik, Stephen Rattigan, Glenn K McConell

JOURNAL OF APPLIED PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2015

Abstract

Inhibition of nitric oxide synthase (NOS) significantly attenuates the increase in skeletal muscle glucose uptake during contraction/exercise, and a greater attenuation is observed in individuals with Type 2 diabetes compared with healthy individuals. Therefore, NO appears to play an important role in mediating muscle glucose uptake during contraction. In this study, we investigated the involvement of neuronal NOSμ (nNOSμ), the main NOS isoform activated during contraction, on skeletal muscle glucose uptake during ex vivo contraction. Extensor digitorum longus muscles were isolated from nNOSμ(-/-) and nNOSμ(+/+) mice. Muscles were contracted ex vivo in a temperature-controlled (30°C) organ b..

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