Long-term measurement of renal cortical and medullary tissue oxygenation and perfusion in unanesthetized sheep
Paolo Calzavacca, Roger G Evans, Michael Bailey, Yugeesh R Lankadeva, Rinaldo Bellomo, Clive N May
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY | AMER PHYSIOLOGICAL SOC | Published : 2015
The role of renal cortical and medullary hypoxia in the development of acute kidney injury is controversial, partly due to a lack of techniques for the long-term measurement of intrarenal oxygenation and perfusion in conscious animals. We have, therefore, developed a methodology to chronically implant combination probes to chronically measure renal cortical and medullary tissue perfusion and oxygen tension (tPO2) in conscious sheep and evaluated their responsiveness and reliability. A transit-time flow probe and a vascular occluder were surgically implanted on the left renal artery. At the same operation, dual fiber-optic probes, comprising a fluorescence optode to measure tPO2 and a laser-D..View full abstract
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Awarded by National Health and Medical Research Council of Australia (NHMRC)
This study was supported by a grant from the National Health and Medical Research Council of Australia (NHMRC; 1050672), and by funding from the Victorian Government Operational Infrastructure Support Grant. C. N. May was supported by a NHMRC research fellowship and P. Calzavacca was supported by an International Postgraduate Student Scholarship from the University of Melbourne.