Journal article
Long-term intermittent hypoxia elevates cobalt levels in the brain and injures white matter in adult mice
SC Veasey, J Lear, Y Zhu, JB Grinspan, DJ Hare, S Wang, D Bunch, PA Doble, SR Robinson
Sleep | Published : 2013
DOI: 10.5665/sleep.3038
Abstract
Study Objectives: Exposure to the variable oxygenation patterns in obstructive sleep apnea (OSA) causes oxidative stress within the brain. We hypothesized that this stress is associated with increased levels of redox-active metals and white matter injury. Design: Participants were randomly allocated to a control or experimental group (single independent variable). Setting: University animal house. Participants: Adult male C57BL/6J mice. Interventions: To model OSA, mice were exposed to long-term intermittent hypoxia (LTIH) for 10 hours/day for 8 weeks or sham intermittent hypoxia (SIH). Measurements and Results: Laser ablation-inductively coupled plasma-mass spectrometry was used to quantita..
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Awarded by National Heart, Lung, and Blood Institute
Funding Acknowledgements
Dr. Veasey and Dr. Lear contributed equally to this work. Some of this research was undertaken while Dr. Robinson was on sabbatical at the Centre for Sleep and Circadian Neurobiology, University of Pennsylvania. The authors acknowledge the support of National Institutes of Health (HL 079555 and HL 096037), the Australian Research Council (DP120102614) and Agilent Technologies, Kennelec Scientific (LP100200254). The authors thank Dr Diane Lim for assisting at the outset of this study and for her thoughtful comments on early drafts of this manuscript. We are also grateful to Professors Ralf Dringen and Allan Pack for their helpful suggestions. Work for this study was performed at University of Technology, Sydney; University of Pennsylvania; Cleveland Clinic; and Monash University.