Journal article

Citrulline Does Not Prevent Skeletal Muscle Wasting or Weakness in Limb-Casted Mice

Daniel J Ham, Tahnee L Kennedy, Marissa K Caldow, Annabel Chee, Gordon S Lynch, Rene Koopman

JOURNAL OF NUTRITION | AMER SOC NUTRITION-ASN | Published : 2015

Abstract

BACKGROUND: Increasing arginine (Arg) availability reduces atrophy in cultured skeletal muscle cells. Supplementation with its metabolic precursor citrulline (Cit) is more effective at improving skeletal muscle Arg concentrations. OBJECTIVE: We tested the hypothesis that Cit supplementation would attenuate skeletal muscle atrophy and loss of function during hindlimb immobilization in mice. METHODS: Male C57BL/6JArc mice underwent 14 d of unilateral hindlimb immobilization/plaster casting and were supplemented with ~0.81 g Cit · kg⁻¹ · d⁻¹ (CIT group) or Ala (ALA group) mixed into their food. The uncasted contralateral limb (internal control) and an uncasted group (CON) served as controls. Mu..

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