Citrulline Does Not Prevent Skeletal Muscle Wasting or Weakness in Limb-Casted Mice
Daniel J Ham, Tahnee L Kennedy, Marissa K Caldow, Annabel Chee, Gordon S Lynch, Rene Koopman
JOURNAL OF NUTRITION | AMER SOC NUTRITION-ASN | Published : 2015
BACKGROUND: Increasing arginine (Arg) availability reduces atrophy in cultured skeletal muscle cells. Supplementation with its metabolic precursor citrulline (Cit) is more effective at improving skeletal muscle Arg concentrations. OBJECTIVE: We tested the hypothesis that Cit supplementation would attenuate skeletal muscle atrophy and loss of function during hindlimb immobilization in mice. METHODS: Male C57BL/6JArc mice underwent 14 d of unilateral hindlimb immobilization/plaster casting and were supplemented with ~0.81 g Cit · kg⁻¹ · d⁻¹ (CIT group) or Ala (ALA group) mixed into their food. The uncasted contralateral limb (internal control) and an uncasted group (CON) served as controls. Mu..View full abstract
Awarded by Ajinomoto Amino Acid Research Program
Supported by the Ajinomoto Amino Acid Research Program grant 3ARP: 09/07.