Journal article

Ultra-high-density 3D DNA arrays within nanoporous biocompatible membranes for single-molecule-level detection and purification of circulating nucleic acids

M Aramesh, O Shimoni, K Fox, TJ Karle, A Lohrmann, K Ostrikov, S Prawer, J Cervenka

NANOSCALE | ROYAL SOC CHEMISTRY | Published : 2015

Abstract

Extracellular nucleic acids freely circulating in blood and other physiologic fluids are important biomarkers for non-invasive diagnostics and early detection of cancer and other diseases, yet difficult to detect because they exist in very low concentrations and large volumes. Here we demonstrate a new broad-range sensor platform for ultrasensitive and selective detection of circulating DNA down to the single-molecule level. The biosensor is based on a chemically functionalized nanoporous diamond-like carbon (DLC) coated alumina membrane. The few nanometer-thick, yet perfect and continuous DLC-coating confers the chemical stability and biocompatibility of the sensor, allowing its direct appl..

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University of Melbourne Researchers

Grants

Awarded by Australian Research Council


Funding Acknowledgements

We acknowledge financial support from the University of Melbourne research and CSIRO top-up scholarships. The authors wish to acknowledge research facility and technical assistance from the Nanostructured Interfaces and Materials Science Group (NIMS) led by Prof. F. Caruso at the department of Chemical and Biomolecular Engineering, the University of Melbourne. We thank Dr P. A. Tran and D. Prakash Biswas at the Tissue Engineering Group at the department of Chemical and Biomolecular Engineering at the University of Melbourne, for cytotoxicity assay. We thank Dr S. Rubanov and Dr R. Curtain at microscopy unit of Bio21 institute for assistance with SEM and TEM imaging. Partial support from CSIRO Science Leadership Scheme and Australian Research Council is acknowledged. This research was supported under Australian Research Council's Discovery Projects funding scheme (project number DE120101100).