Paternal obesity in a rodent model affects placental gene expression in a sex-specific manner
Natalie K Binder, Sally A Beard, Tu'uhevaha J Kaitu'u-Lino, Stephen Tong, Natalie J Hannan, David K Gardner
REPRODUCTION | BIOSCIENTIFICA LTD | Published : 2015
Fetal growth restriction (FGR) is a major obstetric complication stemming from poor placental development. We have previously demonstrated that paternal obesity in mice is associated with impaired embryo development and significantly reduced fetal and placental weights. We hypothesised that the FGR observed in our rodent model of paternal diet-induced obesity is associated with alterations in metabolic, cell signalling and stress pathways. Male C57BL/6 mice were fed either a normal or high-fat diet for 10 weeks before sperm collection for IVF and subsequent embryo transfer. On embryonic day 14, placentas were collected and RNA extracted from both male and female placentas to assess mRNA expr..View full abstract
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Awarded by National Health and Medical Research Council
This work was supported by the University of Melbourne (to D K Gardner), the National Health and Medical Research Council (#1050765 to S Tong, #1062418 to T J Kaitu'u-Lino and #628927 to N J Hannan), the Australian Postgraduate Award (to N K Binder) and the Jasper Loftus-Hills Memorial Award (to N K Binder).