Determining the Subcellular Location of Synthesis and Assembly of the Cell Wall Polysaccharide (1,3;1,4)-beta-D-Glucan in Grasses
Sarah M Wilson, Yin Ying Ho, Edwin R Lampugnani, Allison ML Van de Meene, Melissa P Bain, Antony Bacic, Monika S Doblin
PLANT CELL | AMER SOC PLANT BIOLOGISTS | Published : 2015
The current dogma for cell wall polysaccharide biosynthesis is that cellulose (and callose) is synthesized at the plasma membrane (PM), whereas matrix phase polysaccharides are assembled in the Golgi apparatus. We provide evidence that (1,3;1,4)-β-D-glucan (mixed-linkage glucan [MLG]) does not conform to this paradigm. We show in various grass (Poaceae) species that MLG-specific antibody labeling is present in the wall but absent over Golgi, suggesting it is assembled at the PM. Antibodies to the MLG synthases, cellulose synthase-like F6 (CSLF6) and CSLH1, located CSLF6 to the endoplasmic reticulum, Golgi, secretory vesicles, and the PM and CSLH1 to the same locations apart from the PM. This..View full abstract
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Awarded by Australian Research Council
We thank Cherie Beahan and Marija Todorovic-Glavanic for their technical assistance and Rachel Burton for the T7-tagged CSLF/H constructs. We acknowledge the support of the Australian Research Council for funding to the ARC Centre of Excellence in Plant Cell Walls, a Discovery Project grant (DP110100410), and the Commonwealth Scientific and Research Organization Flagship Collaborative Research Program, provided to the High Fibre Grains Cluster via the Food Futures Flagship. Y.Y.H. acknowledges the support of a Melbourne Research Scholarship.