Journal article
In vivo functional analysis of the Drosophila melanogaster nicotinic acetylcholine receptor Dα6 using the insecticide spinosad
J Somers, J Nguyen, C Lumb, P Batterham, T Perry
Insect Biochemistry and Molecular Biology | Published : 2015
Abstract
The vinegar fly, Drosophila melanogaster, has been used to identify and manipulate insecticide resistance genes. The advancement of genome engineering technology and the increasing availability of pest genome sequences has increased the predictive and diagnostic capacity of the Drosophila model. The Drosophila model can be extended to investigate the basic biology of the interaction between insecticides and the proteins they target. Recently we have developed an in vivo system that permits the expression and study of key insecticide targets, the nicotinic acetylcholine receptors (nAChRs), in controlled genetic backgrounds. Here this system is used to study the interaction between the insecti..
View full abstractGrants
Awarded by Australian Research Council
Funding Acknowledgements
The authors would like to thank the providers of materials that were used in this study; J. Bishof and K. Basler for providing their pUASattB vector, Bloomington Stock centre for fly lines and the Australian Drosophila Biomedical Research Support Facility for handling quarantine and importing flies. Robert T Good for assistance designing CRISPR target. They would also like to acknowledge sources of funding for the project, Australian Research Council Discovery Grant (DP120100788 - PB), Australian Wool Innovation Research Fellowship (TP), J.N. Peters Bequest Fellowship (TP), Australian Postgraduate Award (JS) and Dowd Foundation Scholarship (JS).