Journal article
The genomic and phenotypic diversity of Schizosaccharomyces pombe
DC Jeffares, C Rallis, A Rieux, D Speed, M Převorovský, T Mourier, FX Marsellach, Z Iqbal, W Lau, TMK Cheng, R Pracana, M Mülleder, JLD Lawson, A Chessel, S Bala, G Hellenthal, B O'Fallon, T Keane, JT Simpson, L Bischof Show all
Nature Genetics | Published : 2015
DOI: 10.1038/ng.3215
Abstract
Natural variation within species reveals aspects of genome evolution and function. The fission yeast Schizosaccharomyces pombe is an important model for eukaryotic biology, but researchers typically use one standard laboratory strain. To extend the usefulness of this model, we surveyed the genomic and phenotypic variation in 161 natural isolates. We sequenced the genomes of all strains, finding moderate genetic diversity (π = 3 × 10 -3 substitutions/site) and weak global population structure. We estimate that dispersal of S. pombe began during human antiquity (∼340 BCE), and ancestors of these strains reached the Americas at ∼1623 CE. We quantified 74 traits, finding substantial heritable ph..
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Awarded by European Commission
Funding Acknowledgements
We thank L. Clissold, H. Musk, D. Baker and R Davey for their contributions to sequencing, H. Levin for discussions about transposons, and J. Mao and S. Marguerat for comments on the manuscript. This work Was supported by a Wellcome Trust Senior Investigator Award to TB. (grant 095598/Z/11/Z), by the Welcome Trust to S.B., T.K., J.T.S. and RD., by grant 260801-BIG-IDEA from the European Research Council (ERC) and grant BB/H005854/1 from the Biotechnology and Biological Sciences Research Council (PBS RC) to A.R. and FR, by UK Medical Research Council grant G0901388 to HS, and by a Cancer Research UK Postdoctoral Fellowship to T.M.K.C., by an ERC Starting Grant (SYSGRO) to R.E.C.S., a Welcome Trust PhD studentship to J.L.D.L. and NSW" grant 1;14/K006320/1 to R.E.C.S. and A.C., by a Welcome Trust grant (RG 0937351Z/10/Z) and ERC Starting Grant 260809 to M.R. (MR. is a Welcome Trust Research Career Development and Welk ome-Beit Prize Fellow), by Czech Science Foundation grant P305/12/P040 and Charles University grant UNCE 204013 to M.P. and by Cancer Research UK to L.J. and J.H.