Journal article

Critical role of thrombopoietin in maintaining adult quiescent hematopoietic stem cells

Hong Qian, Natalija Buza-Vidas, Craig D Hyland, Christina T Jensen, Jennifer Antonchuk, Robert Mansson, Lina A Thoren, Marja Ekblorn, Warren S Alexander, Sten Eirik W Jacobsen

Cell Stem Cell | CELL PRESS | Published : 2007

Abstract

The role of cytokines in regulation of hematopoietic stem cells (HSCs) remains poorly understood. Herein we demonstrate that thrombopoietin (THPO) and its receptor, MPL, are critically involved in postnatal steady-state HSC maintenance, reflected in a 150-fold reduction of HSCs in adult Thpo(-/-) mice. Further, whereas THPO and MPL proved not required for fetal HSC expansion, HSC expansion posttransplantation was highly MPL and THPO dependent. The distinct role of THPO in postnatal HSC maintenance is accompanied by accelerated HSC cell-cycle kinetics in Thpo(-/-) mice and reduced expression of the cyclin-dependent kinase inhibitors p57(Kip2) and p19(INK4D) as well as multiple Hox transcripti..

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University of Melbourne Researchers