Journal article

The role of osmiophilic bodies and Pfg377 expression in female gametocyte emergence and mosquito infectivity in the human malaria parasite Plasmodium falciparum

Tania F de Koning-Ward, Anna Olivieri, Lucia Bertuccini, Andrew Hood, Francesco Silvestrini, Konstantinos Charvalias, Pedro Berzosa Diaz, Grazia Camarda, Terry F McElwain, Tony Papenfuss, Julie Healer, Lucilla Baldassarri, Brendan S Crabb, Pietro Alano, Lisa C Ranford-Cartwright



Osmiophilic bodies are membrane-bound vesicles, found predominantly in Plasmodium female gametocytes, that become progressively more abundant as the gametocyte reaches full maturity. These vesicles lie beneath the subpellicular membrane of the gametocyte, and the release of their contents into the parasitophorous vacuole has been postulated to aid in the escape of gametocytes from the erythrocyte after ingestion by the mosquito. Currently, the only protein known to be associated with osmiophilic bodies in Plasmodium falciparum is Pfg377, a gametocyte-specific protein expressed at the onset of osmiophilic body development. Here we show by targeted gene disruption that Pfg377 plays a fundament..

View full abstract