Clearance of Measles Virus from Persistently Infected Cells by Short Hairpin RNA
Michael Zinke, Sabine Kendl, Katrin Singethan, Markus Fehrholz, Dajana Reuter, Linda Rennick, Marco J Herold, Juergen Schneider-Schaulies
JOURNAL OF VIROLOGY | AMER SOC MICROBIOLOGY | Published : 2009
Subacute sclerosing panencephalitis (SSPE) is a demyelinating central nervous system disease caused by a persistent measles virus (MV) infection of neurons and glial cells. There is still no specific therapy available, and in spite of an intact innate and adaptive immune response, SSPE leads inevitably to death. In order to select effective antiviral short interfering RNAs (siRNAs), we established a plasmid-based test system expressing the mRNA of DsRed2 fused with mRNA sequences of single viral genes, to which certain siRNAs were directed. siRNA sequences were expressed as short hairpin RNA (shRNA) from a lentiviral vector additionally expressing enhanced green fluorescent protein (EGFP) as..View full abstract
We thank the Deutsche Forschungsgemeinschaft for financial support.